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biotinylated ulex europaeus agglutinin i (uea i)  (Vector Laboratories)


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    Structured Review

    Vector Laboratories biotinylated ulex europaeus agglutinin i (uea i)
    Biotinylated Ulex Europaeus Agglutinin I (Uea I), supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 517 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ulex+europaeus+agglutinin/Biotinylated+Ulex+Europaeus+Agglutinin+I+(UEA+I)/custom%40b-1065%4042537647
    Average 96 stars, based on 517 article reviews
    biotinylated ulex europaeus agglutinin i (uea i) - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Electrical stimulation directs formation of perfused vasculature in engineered tissues
    Article Snippet: .. After fixation, the samples were washed with 1 x PBS, blocked with 3 % BSA solution and stained using 1:200 solution of Ulex Europaeus Agglutinin I (UEA I), DyLight 649 (Vector Labs). .. Confocal images of whole perfused grafts were performed using Zeiss LSM.

    Article Title: ETV2 mediated differentiation of human pluripotent stem cells results in functional endothelial cells for engineering advanced vascularized microphysiological models
    Article Snippet: The expressions of CD31, CD34, VEGFR2, and UEA-I were tested by Flow cytometry (BD LSR-II). .. Cells were stained with PE anti-human CD31 antibody (Biolegend, 1:100), Ulex Europaeus Agglutinin I DyLight 649 (Vector Laboratories, 1:200), APC anti-human CD34 antibody (Biolegend, 1:100), or PE anti-human CD309 antibody (Biolegend, 1:100) on ice for 15 min. ..

    Article Title: Milk-derived casein glycomacropeptide improves colonic mucus function under Western-style diet feeding in a sialylation-dependent manner.
    Article Snippet: .. After mounting the tissue sample on the perfusion chamber, the epithelium was stained with Syto 9 (Thermo Fisher, 1:500 in Kreb’s-mannitol buffer), and the mucus layer was stained with Wheat Germ Agglutinin (Thermo Fisher, 1:20 in Kreb’s-mannitol buffer) and Ulex Europaeus Agglutinin I (Vector Laboratories, 1:20 in Kreb’smannitol buffer). .. After 15 min of incubation on ice, tissue was washed with Kreb’s-mannitol buffer and the mucus was overlaid with 1μmfluorescent microbeads (Thermo Fisher, 1:20 in Kreb’s-mannitol buffer).

    other:

    Article Title: Comparative phenotyping of surface markers and glycans in murine and human platelet-derived extracellular vesicles
    Article Snippet: Ricinus communis agglutinin 1, succinylated wheat germ agglutinin, Sambucus nigra , Maackia amurensis , concanavalin A, and Ulex europaeus agglutinin (all fluorescein-conjugated) were from Vector Laboratories.

    Incubation:

    Article Title: Vessel-on-Chip model of the microcirculation in Abdominal aortic aneurysms
    Article Snippet: .. To visualize vascular structures, VoCs were incubated with Ulex Europaeus Agglutinin I (UEA-I, DyLight 649, DL-1068-1, Vector Laboratories; dilution 1:600) for 1 hour under standard incubation conditions (37°C, 5% CO2). .. Live-cell confocal imaging was then performed using a Nikon AXR microscope equipped with environmental control.

    Article Title: Deciphering human heart failure with preserved ejection fraction (HFpEF) at single cell resolution
    Article Snippet: .. After permeabilization with 0.2% Triton X-100 (ThermoFisher T8787), sections were incubated in a blocking solution for one hour at room temperature (2% Donkey Serum (abcam ab7475), 3% BSA (Sigma-Aldrich A1595), 0.2% Triton X-100 in DPBS (130225 PAA Laboratories)) and incubated with a ROCK1 antibody (abcam Alexa Fluor® 488 Anti-ROCK1 [EPR638Y], 1:50) and Ulex Europaeus Agglutinin I (Vector Laboratories B-1065-2, 1:100) in blocking solution at 4°C, overnight. .. After washing, streptavidin-conjugated Alexa Fluor® 555 (ThermoFisher S21381, 1:200) was added, slides were washed and embedded in Fluoromount-G (Invitrogen 00-4958-0) with Hoechst 33342 (Life Technologies H3570, 1300).

    Whole Genome Amplification:

    Article Title: Lipopolysaccharide Detection with Glycan-Specific Lectins—a Nonspecific Binding Approach Applied to Surface Plasmon Resonance
    Article Snippet: Phosphate buffer solution pH 7.4 (1X PBS) was purchased from Corning (Corning, US). .. Biotinylated lectins Concanavalin (ConA), Glycine max agglutinin (SBA), Triticum vulgaris agglutinin (WGA), Dolichos biflorus agglutinin (DBA), Ulex europaeus agglutinin (UEA I), Ricinus communis agglutinin (RCA), and Arachis hypogea agglutinin (PNA) were purchased from Vector Laboratories (Newark, US). .. Phenol extracted LPS from E. coli O55:B5 (CDC 1644-70), E. coli O111:B4 (private source), K. pneumoniae (ATCC 15830), P. aeruginosa (ATCC 27316), and S. enterica (ATCC 7823) were purchased from Sigma-Aldrich (Oakville, Canada).

    Article Title: Fluorescence Lifetime Imaging Microscopy (FLIM) visualizes internalization and biological impact of nanoplastics in live intestinal organoids
    Article Snippet: Imaging was performed in phenol red-free DMEM (Sigma-Aldrich, D5030), supplemented with 10 mM glucose (G8270, Sigma-Aldrich), 1 mM pyruvate (11360-070, Gibco, Belgium), 2 mM GlutaMAX TM (35050-038, Gibco, Belgium) and 10 mM HEPES-Na pH 7.2 buffer (15630-080, Gibco, Belgium) referred as imaging media (IM). .. Alexa Fluor 488-conjugated Wheat Germ Agglutinin (WGA; W11261, Invitrogen, Belgium), Fluorescein-labeled Ulex europaeus Agglutinin I (UEA I; VEC.FL-1061-5, Vector Laboratories, Belgium), Nile Red (72485-100MG, Sigma-Aldrich, Belgium), MitoTracker Green (M7514, Invitrogen, Belgium), MitoTracker Red (M22425, Invitrogen, Belgium), Tetramethylrhodamine, methyl ester (TMRM) (T668, Invitrogen, Belgium), phalloidin-Alexa Fluor 546 (Invitrogen, A22283) Texas Red-X phalloidin (T7471, Invitrogen, Belgium). ..

    Blocking Assay:

    Article Title: Deciphering human heart failure with preserved ejection fraction (HFpEF) at single cell resolution
    Article Snippet: .. After permeabilization with 0.2% Triton X-100 (ThermoFisher T8787), sections were incubated in a blocking solution for one hour at room temperature (2% Donkey Serum (abcam ab7475), 3% BSA (Sigma-Aldrich A1595), 0.2% Triton X-100 in DPBS (130225 PAA Laboratories)) and incubated with a ROCK1 antibody (abcam Alexa Fluor® 488 Anti-ROCK1 [EPR638Y], 1:50) and Ulex Europaeus Agglutinin I (Vector Laboratories B-1065-2, 1:100) in blocking solution at 4°C, overnight. .. After washing, streptavidin-conjugated Alexa Fluor® 555 (ThermoFisher S21381, 1:200) was added, slides were washed and embedded in Fluoromount-G (Invitrogen 00-4958-0) with Hoechst 33342 (Life Technologies H3570, 1300).



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    Characterization of endothelial progenitor cells (EPCs) and transfection efficacy of small interfering RNA targeting transforming growth factor-β1 (si-TGF-β1). a) was the immunofluorescence result of cluster of differentiation (CD)34 and vascular endothelial growth factor receptor 2 (VEGFR2). b) EPCs could simultaneously absorb DiI-labelled acetylated low-density lipoprotein (Dil-Ac-LDL) and <t>fluorescein</t> <t>isothiocyanate-labeled</t> Ulex <t>europaeus</t> <t>agglutinin</t> <t>I</t> (FITC-UEA-I). Scale bar: 100 μm. c) Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to validate the silencing effect of si-TGF-β1 #1, #2 and #3. N = 5/group. d) Western blot was used to validate the silencing effect of si-TGF-β1 #1, #2 and #3. N = 5/group. Each value was presented as the mean (SD). ***p < 0.001 vs the si-TGF-β1 negative control (NC) group; ##p < 0.01, ###p < 0.001 vs the si-TGF-β1 #2 group.
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    ( a )Representative images of Epi Ctrl and Epi ΔBmal1 distal colon stained <t>for</t> <t>UEA-1</t> (violet) and MALII (yellow) to label fucosylated and sialylated mucins, respectively. Shown are samples collected at ZT0 and ZT12, arrowheads indicate mucus thickness. (Scale bar = 20μm). ( b ) UEA-1+ mucus thickness in the distal colon is significantly higher at ZT12 in Epi Ctrl but lower at both times in Epi ΔBmal1 (**p≤0.01). ( c ) MALII+ mucus thickness in the distal colon is significantly higher at ZT12 in Epi Ctrl (*p≤ 0.05), with a significant increase of thickness in Epi ΔBmal1 at ZT0 (*p<0.05). Note, that Epi ΔBmal1 does not show a time-of-day dependent change in MALII. ( d ) UEA-1+ goblet cell area in distal colon crypts s significantly higher at ZT12 in Epi Ctrl (**p≤0.05). While not significantly different from controls, Epi ΔBmal1 do not show this time-of-day change in UEA-1+ mucus thickness. ( e ) MALII+ goblet cell area in distal colon crypts is significantly increased in Epi ΔBmal1 at ZT0 (*p≤0.05).
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    ( a )Representative images of Epi Ctrl and Epi ΔBmal1 distal colon stained <t>for</t> <t>UEA-1</t> (violet) and MALII (yellow) to label fucosylated and sialylated mucins, respectively. Shown are samples collected at ZT0 and ZT12, arrowheads indicate mucus thickness. (Scale bar = 20μm). ( b ) UEA-1+ mucus thickness in the distal colon is significantly higher at ZT12 in Epi Ctrl but lower at both times in Epi ΔBmal1 (**p≤0.01). ( c ) MALII+ mucus thickness in the distal colon is significantly higher at ZT12 in Epi Ctrl (*p≤ 0.05), with a significant increase of thickness in Epi ΔBmal1 at ZT0 (*p<0.05). Note, that Epi ΔBmal1 does not show a time-of-day dependent change in MALII. ( d ) UEA-1+ goblet cell area in distal colon crypts s significantly higher at ZT12 in Epi Ctrl (**p≤0.05). While not significantly different from controls, Epi ΔBmal1 do not show this time-of-day change in UEA-1+ mucus thickness. ( e ) MALII+ goblet cell area in distal colon crypts is significantly increased in Epi ΔBmal1 at ZT0 (*p≤0.05).
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    ( a )Representative images of Epi Ctrl and Epi ΔBmal1 distal colon stained <t>for</t> <t>UEA-1</t> (violet) and MALII (yellow) to label fucosylated and sialylated mucins, respectively. Shown are samples collected at ZT0 and ZT12, arrowheads indicate mucus thickness. (Scale bar = 20μm). ( b ) UEA-1+ mucus thickness in the distal colon is significantly higher at ZT12 in Epi Ctrl but lower at both times in Epi ΔBmal1 (**p≤0.01). ( c ) MALII+ mucus thickness in the distal colon is significantly higher at ZT12 in Epi Ctrl (*p≤ 0.05), with a significant increase of thickness in Epi ΔBmal1 at ZT0 (*p<0.05). Note, that Epi ΔBmal1 does not show a time-of-day dependent change in MALII. ( d ) UEA-1+ goblet cell area in distal colon crypts s significantly higher at ZT12 in Epi Ctrl (**p≤0.05). While not significantly different from controls, Epi ΔBmal1 do not show this time-of-day change in UEA-1+ mucus thickness. ( e ) MALII+ goblet cell area in distal colon crypts is significantly increased in Epi ΔBmal1 at ZT0 (*p≤0.05).
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    ( a )Representative images of Epi Ctrl and Epi ΔBmal1 distal colon stained <t>for</t> <t>UEA-1</t> (violet) and MALII (yellow) to label fucosylated and sialylated mucins, respectively. Shown are samples collected at ZT0 and ZT12, arrowheads indicate mucus thickness. (Scale bar = 20μm). ( b ) UEA-1+ mucus thickness in the distal colon is significantly higher at ZT12 in Epi Ctrl but lower at both times in Epi ΔBmal1 (**p≤0.01). ( c ) MALII+ mucus thickness in the distal colon is significantly higher at ZT12 in Epi Ctrl (*p≤ 0.05), with a significant increase of thickness in Epi ΔBmal1 at ZT0 (*p<0.05). Note, that Epi ΔBmal1 does not show a time-of-day dependent change in MALII. ( d ) UEA-1+ goblet cell area in distal colon crypts s significantly higher at ZT12 in Epi Ctrl (**p≤0.05). While not significantly different from controls, Epi ΔBmal1 do not show this time-of-day change in UEA-1+ mucus thickness. ( e ) MALII+ goblet cell area in distal colon crypts is significantly increased in Epi ΔBmal1 at ZT0 (*p≤0.05).
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    ( a )Representative images of Epi Ctrl and Epi ΔBmal1 distal colon stained <t>for</t> <t>UEA-1</t> (violet) and MALII (yellow) to label fucosylated and sialylated mucins, respectively. Shown are samples collected at ZT0 and ZT12, arrowheads indicate mucus thickness. (Scale bar = 20μm). ( b ) UEA-1+ mucus thickness in the distal colon is significantly higher at ZT12 in Epi Ctrl but lower at both times in Epi ΔBmal1 (**p≤0.01). ( c ) MALII+ mucus thickness in the distal colon is significantly higher at ZT12 in Epi Ctrl (*p≤ 0.05), with a significant increase of thickness in Epi ΔBmal1 at ZT0 (*p<0.05). Note, that Epi ΔBmal1 does not show a time-of-day dependent change in MALII. ( d ) UEA-1+ goblet cell area in distal colon crypts s significantly higher at ZT12 in Epi Ctrl (**p≤0.05). While not significantly different from controls, Epi ΔBmal1 do not show this time-of-day change in UEA-1+ mucus thickness. ( e ) MALII+ goblet cell area in distal colon crypts is significantly increased in Epi ΔBmal1 at ZT0 (*p≤0.05).
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    Image Search Results


    Characterization of endothelial progenitor cells (EPCs) and transfection efficacy of small interfering RNA targeting transforming growth factor-β1 (si-TGF-β1). a) was the immunofluorescence result of cluster of differentiation (CD)34 and vascular endothelial growth factor receptor 2 (VEGFR2). b) EPCs could simultaneously absorb DiI-labelled acetylated low-density lipoprotein (Dil-Ac-LDL) and fluorescein isothiocyanate-labeled Ulex europaeus agglutinin I (FITC-UEA-I). Scale bar: 100 μm. c) Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to validate the silencing effect of si-TGF-β1 #1, #2 and #3. N = 5/group. d) Western blot was used to validate the silencing effect of si-TGF-β1 #1, #2 and #3. N = 5/group. Each value was presented as the mean (SD). ***p < 0.001 vs the si-TGF-β1 negative control (NC) group; ##p < 0.01, ###p < 0.001 vs the si-TGF-β1 #2 group.

    Journal: Bone & Joint Research

    Article Title: Naringin targets TGF-β1-mediated angiogenesis to enhance the osteogenic effect of induced membrane

    doi: 10.1302/2046-3758.155.BJR-2025-0412.R1

    Figure Lengend Snippet: Characterization of endothelial progenitor cells (EPCs) and transfection efficacy of small interfering RNA targeting transforming growth factor-β1 (si-TGF-β1). a) was the immunofluorescence result of cluster of differentiation (CD)34 and vascular endothelial growth factor receptor 2 (VEGFR2). b) EPCs could simultaneously absorb DiI-labelled acetylated low-density lipoprotein (Dil-Ac-LDL) and fluorescein isothiocyanate-labeled Ulex europaeus agglutinin I (FITC-UEA-I). Scale bar: 100 μm. c) Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to validate the silencing effect of si-TGF-β1 #1, #2 and #3. N = 5/group. d) Western blot was used to validate the silencing effect of si-TGF-β1 #1, #2 and #3. N = 5/group. Each value was presented as the mean (SD). ***p < 0.001 vs the si-TGF-β1 negative control (NC) group; ##p < 0.01, ###p < 0.001 vs the si-TGF-β1 #2 group.

    Article Snippet: For functional verification, EPCs were exposed to DiI-labeled acetylated low-density lipoprotein(Dil-Ac-LDL; Thermo Fisher Scientific, USA) and fluorescein isothiocyanate-labeled Ulex europaeus agglutinin I (FITC-UEA-I; Thermo Fisher Scientific) in a dark environment.

    Techniques: Transfection, Small Interfering RNA, Immunofluorescence, Labeling, Reverse Transcription, Polymerase Chain Reaction, Quantitative RT-PCR, Western Blot, Negative Control

    ( a )Representative images of Epi Ctrl and Epi ΔBmal1 distal colon stained for UEA-1 (violet) and MALII (yellow) to label fucosylated and sialylated mucins, respectively. Shown are samples collected at ZT0 and ZT12, arrowheads indicate mucus thickness. (Scale bar = 20μm). ( b ) UEA-1+ mucus thickness in the distal colon is significantly higher at ZT12 in Epi Ctrl but lower at both times in Epi ΔBmal1 (**p≤0.01). ( c ) MALII+ mucus thickness in the distal colon is significantly higher at ZT12 in Epi Ctrl (*p≤ 0.05), with a significant increase of thickness in Epi ΔBmal1 at ZT0 (*p<0.05). Note, that Epi ΔBmal1 does not show a time-of-day dependent change in MALII. ( d ) UEA-1+ goblet cell area in distal colon crypts s significantly higher at ZT12 in Epi Ctrl (**p≤0.05). While not significantly different from controls, Epi ΔBmal1 do not show this time-of-day change in UEA-1+ mucus thickness. ( e ) MALII+ goblet cell area in distal colon crypts is significantly increased in Epi ΔBmal1 at ZT0 (*p≤0.05).

    Journal: bioRxiv

    Article Title: Epithelial function of the circadian clock gene, Bmal1 , in regulating the mucosa

    doi: 10.64898/2026.04.15.718752

    Figure Lengend Snippet: ( a )Representative images of Epi Ctrl and Epi ΔBmal1 distal colon stained for UEA-1 (violet) and MALII (yellow) to label fucosylated and sialylated mucins, respectively. Shown are samples collected at ZT0 and ZT12, arrowheads indicate mucus thickness. (Scale bar = 20μm). ( b ) UEA-1+ mucus thickness in the distal colon is significantly higher at ZT12 in Epi Ctrl but lower at both times in Epi ΔBmal1 (**p≤0.01). ( c ) MALII+ mucus thickness in the distal colon is significantly higher at ZT12 in Epi Ctrl (*p≤ 0.05), with a significant increase of thickness in Epi ΔBmal1 at ZT0 (*p<0.05). Note, that Epi ΔBmal1 does not show a time-of-day dependent change in MALII. ( d ) UEA-1+ goblet cell area in distal colon crypts s significantly higher at ZT12 in Epi Ctrl (**p≤0.05). While not significantly different from controls, Epi ΔBmal1 do not show this time-of-day change in UEA-1+ mucus thickness. ( e ) MALII+ goblet cell area in distal colon crypts is significantly increased in Epi ΔBmal1 at ZT0 (*p≤0.05).

    Article Snippet: Sections were incubated with biotinylated MAL-II (B-1265-1, Vector Laboratories; 3μg/mL in 1%BSA in PBS) overnight at 4°C, washed in PBS, then incubated with UEA-1 rhodamine (RL-1062-2, Vector Laboratories; 2μg/mL) and streptavidin-FITC (405201, BioLegend; 5μg/mL) in 1% BSA in PBS for 1 hr at room temperature in the dark.

    Techniques: Staining